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SOLVED: The unfolding, or denaturation, of a biological macromolecule may be brought about by treatment with substances, called denaturants, that disrupt the intermolecular interactions responsible for the native three-dimensional conformation of the polymer. For example, urea, CO(NH2)2, competes for NH and CO groups and interferes with hydrogen bonding in a polypeptide. In a theoretical study of a protein, the temperature-composition diagram shown in Fig. 6.47 was obtained. It shows three struc
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