The dystrophin gene, which is mutated in Duchenne muscular dystrophy, is the largest human gene. A full spectrum of the gene transcripts has not been fully elucidated yet, although two cryptic exons ...

PCR amplification was performed in a total volume of 10 μl, containing 1 μl cDNA, 1 μl 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U ExTaq polymerase (Takara Bio, Inc.), 500 nM each ...

Within multi-exon genes, after introns are removed, the exons must be spliced back together. Splice-enhancer domains (exon sequences near the intron–exon boundary) help to ensure that this happens ...

Pre-mRNA splicing in a subset of human short introns is governed by a distinct mechanism involving a new splicing factor, new research finds. The interrupted non-coding regions in pre-mRNAs, termed ...

In the genome, genes that code for protein are often divided into sections called exons, which are separated by spacers called introns. When a region of DNA is transcribed into messenger RNA (mRNA) or ...